<thead id="wtvt8"></thead>

      <label id="wtvt8"></label>
        1. <li id="wtvt8"><big id="wtvt8"></big></li><span id="wtvt8"><optgroup id="wtvt8"></optgroup></span>
            国产黑色丝袜在线播放,97视频精品全国免费观看,日韩精品中文字幕有码,在线播放深夜精品三级,免费AV片在线观看网址,福利一区二区在线观看,亚洲深夜精品在线观看,2019亚洲午夜无码天堂

            武漢原生原代生物醫(yī)藥科技有限公司

            PriCells: Isolation and Culture of Human Brain Tumor Stem Cell

            時(shí)間:2021-11-10 閱讀:230
            分享:

            PriCells: Isolation and Culture of Human Brain Tumor Stem Cell                             

            The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacity for proliferation, self-renewal, and differentiation. These cells represents a minority of the tumor cell population and are identified by expression of the cell surface marker the positive CD133. These positive CD133 cells, termed the brain tumor stem cells (BTSCs), which are the expression of neural differentiation markers, and are necessary for the proliferation and self-renewal of the tumor in culture.
            1. Tumor samples were obtained from the informed consenting patients.
            2. Tumors were washed, acutely dissociated in oxygenated artificial cerebrospinal fluid and subject to enzymatic dissociation.
            3. Tumor cells were then resuspended in tumor sphere medium (TSM)
                • Serum-free neural stem cell medium
                • Human recombinant EGF (20 ng/ml)
                • bFGF (20 ng/ml)
                • Leukemia inhibitory factor (10 ng/ml)
                • Neuronal Survival Factor (NSF) (10 ng/ml)
                • N-acetylcysteine (60 μg/ml)
            4. Plated at a density of 3 × 106 live cells/60-mm plate.
            5. RBCs were removed using lympholyte-M.
            6. After primary sphere formation was noted, sphere cells were dissociated and plated in 96-well microwell plates in 0.2 ml volumes of TSM.
            7. Final cell dilutions ranged from 200 cells/well to 1 cell/well in 0.2-ml volumes.
            8. Cultures were fed 0.025 ml of TSM every 2 days until day 7, when the percentage of wells not containing spheres for each cell plating density was calculated and plotted against the number of cells per well.
            9. Regression lines were plotted and x-intercept values calculated, which represent the number of cells required to form at least 1 tumor sphere in every well.
            10. CD133-adherent tumor cells were trypsinized before collection for assays.
            11. For primary sphere formation assays, this analysis was performed on the entire acutely dissociated tumor cell population on day 0 to quantify stem cell frequency within the tumor.
            12. Cells were plated in 96-well microwell plates in 0.1-ml volumes of SFM supplemented with growth factors, at a density of 1000 cells/well.
            13. Cell proliferation assays were performed on days 0, 3 5, and 7 postplating using the Roche 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based Colorimetric Assay Cell Proliferation kit 1.
            14. Quantification of viable cells through reading of UV absorption spectrums at 575 nm was performed on a Versamax microplate reader.
            15. Two days after primary culture, cells were plated onto glass coverslips coated in poly-L-ornithine in medium with 10% FBS in individual wells of a 24-well culture plate.
            16. Cells were fed with FBS-supplemented medium every 2 days, and coverslips were processed 7 days after plating using immunocytochemistry.

            會(huì)員登錄

            ×

            請(qǐng)輸入賬號(hào)

            請(qǐng)輸入密碼

            =

            請(qǐng)輸驗(yàn)證碼

            收藏該商鋪

            X
            該信息已收藏!
            標(biāo)簽:
            保存成功

            (空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

            常用:

            提示

            X
            您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
            撥打電話(huà) 產(chǎn)品分類(lèi)
            在線(xiàn)留言
            主站蜘蛛池模板: 亚洲av成人免费在线| 亚洲AV国产福利精品在现观看| 亚洲一区二区三区啪啪| 国产国产午夜福利视频| 精品国产精品午夜福利| 一本精品99久久精品77| 日本边添边摸边做边爱喷水| 国产资源精品中文字幕| 午夜高清福利在线观看| 亚洲成在人线AV品善网好看| 欧美白妞大战非洲大炮| 亚洲一区二区偷拍精品| 亚洲一区二区三区啪啪| 岛国岛国免费v片在线观看| 久久精品国产蜜臀av| 亚洲精品漫画一二三区| 国产午夜精品理论大片| 高清中文字幕国产精品| 亚洲 一区二区 在线| 男女激情一区二区三区| 日夜啪啪一区二区三区| 亚洲av日韩在线资源| 亚洲色拍拍噜噜噜最新网站| 日韩有码国产精品一区| 精品中文字幕人妻一二| 国产精品自在线拍国产手机版| 日韩中文字幕精品人妻| 福利一区二区在线观看| 亚洲最大成人在线播放| 国产福利在线观看免费第一福利 | 野外做受三级视频| 国产成人不卡一区二区| 好紧好滑好湿好爽免费视频| 爱性久久久久久久久| 久久月本道色综合久久| 中文午夜乱理片无码| 国内精品久久人妻无码不卡| 少妇高潮喷水正在播放| 老师扒下内裤让我爽了一夜| 东京一本一道一二三区| 国产破外女出血视频|