LB自誘導(dǎo)培養(yǎng)基LB Broth (Auto Induction Medium)產(chǎn)品介紹選購(gòu)指南

大腸桿菌自誘導(dǎo)培養(yǎng)基適用于由功能性的lac操縱子(包括lac Y和lac Z)控制的表達(dá)系統(tǒng)，如PET系列。表達(dá)菌種可用BL21(DE3)。大腸桿菌自誘導(dǎo)培養(yǎng)基(E.coli Auto-Induction Medium)是在LB培養(yǎng)基的基礎(chǔ)上進(jìn)行改進(jìn)優(yōu)化配方，可用于lac調(diào)控表達(dá)的大腸桿菌菌株培養(yǎng)及蛋白表達(dá)。含有多種營(yíng)養(yǎng)物質(zhì)并額外添加微量元素、氨基酸等，碳源梯度設(shè)計(jì)既能滿足菌體生長(zhǎng)需求，同時(shí)能實(shí)現(xiàn)外源蛋白在細(xì)菌達(dá)到飽和生長(zhǎng)期后的自動(dòng)誘發(fā)。同等產(chǎn)量相比傳統(tǒng)LB使用更少培養(yǎng)體積，無需密切監(jiān)控菌液密度的過程(測(cè)OD)，且無需額外添加IPTG。本品同樣適用于多克隆表達(dá)，高通量選。產(chǎn)品為粉末形式，500g/瓶，按實(shí)驗(yàn)室已經(jīng)習(xí)慣的常規(guī)LB培養(yǎng)基樣配制，配制方便。

LB自誘導(dǎo)培養(yǎng)基LB Broth (Auto Induction Medium)產(chǎn)品介紹選購(gòu)指南

用于制備不含微量元素的LB培養(yǎng)基的固體粉末，添加有葡萄糖和α-乳糖，以便在大腸桿菌中通過IPTG誘導(dǎo)的啟動(dòng)子自動(dòng)誘導(dǎo)蛋白表達(dá)。

Quantity: 500g

Appearance: Beige powder. Autoclaved medium should be amber.

Storage: 2oC – 25oC. When not in use, keep container closed to avoid hydration.

Preparation:
Add 34,85g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121oC for 15 to 20 minutes. Store at 2oC to 8oC.

Formulation (g/L)
Tryptone: 10,00
Yeast Extract: 5,00
MgSO4: 0,15
(NH4) 2SO4: 3,30
KH2PO4: 6,80
Na2HPO4: 7,10
Glucose 0,50
Alpha Lactose 2,00
Final pH (25oC): 7,0 ± 0,2